Fig. 5

A Expert annotated regions in the Human DLPFC dataset. B log library size for each spot shows a significant drop in library sizes in the white matter region (WM). C Normalization using different approaches is unable to recover the expression of a marker gene, MOBP, of oligodendrocytes that are enriched in the white matter region. Not normalizing results in the contradictory inference of exclusion of this gene from the white matter. SpaNorm detects this signal as indicated by the mean estimate of this gene, and the effect is more evident when the percentile-invariant adjusted count (PAC) are scaled up by a factor of 4 (sf = 4) during adjustment